Specialist in NGS Techniques

Località: 

Rozzano, IT, 20089

                                                                                         

The Humanitas Clinical Center, IRCCS is seeking a motivated candidate to join our Humanitas Genomic Facility (HuGe). The mission of the HuGe is to facilitate the development and implementation of innovative methods and emerging sequencing technologies that directly aid precision medicine efforts. The successful candidate will report directly to the Chair of the Facility Management.

As part of a larger group that uses next-generation sequencing approaches, we are recruiting a new colleague graduate or a tech with experience to work on exciting projects derived from the collaborative interactions with many different scientific groups within Humanitas Clinical Center as well outside the hospital.

The candidate will be responsible for setting up and maintaining a state-of-the-art next-generation sequencing platforms (mainly Illumina-based), to assist researchers in both genome-wide and gene-focused level to characterize samples at genomic (genotyping, somatic mutations detection, copy number analysis), transcriptomic (bulk and single-cell RNA-seq), and epi-genomic (Methylation array, Methyl-seq, miRNA-seq, CHIP-seq., ATAC-seq.) levels.

The candidate will be able to generate library starting from a variety of bio-samples, including bulk tissues (snap frozen or FFPE), liquid biopsies (serum or plasma) and microbiota. Strong emphasis will be put on single-cell for spatially and temporally resolved single cell analytics.

As new technologies will be routinely selected and developed among the different scientific areas of basic science and translational medicine, the candidate will also be expected to contribute to the development of new protocols and the rapid standardization/implementation of new approaches for bespoke needs.

 

Skills and responsibilities

The candidate will:

  • be responsible for maintaining the state-of-the-art next-generation sequencing platforms and protocols.
  • Organize the work independently.
  • Support and supervise other groups in sample preparation as well as in quality check.
  • Cooperate with other groups to implement novel technologies.
  • Constantly optimize protocols and maximize cost-effectiveness.
  • Participate actively in the facility activity.
  • Report regularly on progress to the HuGe chair.
  • Build effective links and collaborations

Job requirements

The candidate will have:

  • Bachelor's Degree or higher in molecular biology, genetics, biochemistry or a related field of science.
  • >3 years of hands-on experience in the use of sequencing technologies.
  • Experience in genomic DNA/RNA extraction and quality control using kits and/or automated solutions from difference sources.
  • Experience with library preparation and sequencing on Illumina platforms (MySeq, NextSeq 550, NexSeq2000, Novaseq6000).
  • Previous equivalent experience in a Genomics Core Facility (not mandatory).
  • Documented experience in operating and developing NGS protocols starting from any kind of material (solid and liquid biopsy, snap frozen or FFPE).
  • Excellent laboratory skills and trouble-shooting.
  • Strong communication and interpersonal skills. Proven ability to work in a research laboratory both autonomously and as part of a team to drive projects forward.
  • Written and spoken English.
  • Proven communication, team building and problem-solving skills.

 

The role will be based in Rozzano