Laboratory Technician Molecular-Cell Biology


Rozzano, IT, 20089


The research group of Precision Medicine and Oncognomics led by Prof. Salvatore Piscuoglio ( will be based al the Humanitas University, Milan, Italy, from January 2024 is seeking three highly motivated Laboratory Technicians in Molecular Biology/ Cell Biology (100%) to join our team of bioinformaticians, molecular biologists, pathologists, and clinical fellows.


The candidates will develop, optimize and apply cutting-edge cellular/ molecular methods to generate 3D in vitro and ex vivo tumor models that adequately recapitulate the tumor architecture and mimic the cell-to-cell interactions within the tissue microenvironment. The specific tasks will include the generation and maintenance of patient-derived tumor organoids, patient-derived tumor fragments, fibroblasts, endothelial cells, Peripheral Blood Mononuclear Cells (PBMCs), and Tumor Infiltrating Lymphocytes (TILs). 


Main responsibilities include

  • processing patient-derived samples according to pre-established guidelines (e.g., tissue dissociation, isolation of PBMCs/ TILs, endothelial cells, sample freezing and storage, nucleic acids and protein extraction, and patient-derived tumor organoid/ fragment generation and maintenance)
  • generation and maintenance of cell cultures and execution of experiments on cell cultures
  • updating the database of patient samples and reagents
  • documenting the experiments
  • providing general support for lab maintenance within the scope of this project



  • "Laboratory Technician" degree or a Bachelor/Master degree in molecular biology, biotechnology or a related discipline
  • excellent technical expertise in molecular/ cell biology techniques
  • hands-on experience handling and processing patient samples
  • hands-on experience with cell culture and flow cytometry techniques is a must
  • experience in primary cell culture and 3D cultures is highly desirable
  • technical expertise in organoid generation and maintenance is a plus
  • once trained, the ability to work independently
  • organized and detail-oriented; ability to maintain accurate documentation of experiments and sample information
  • collaborative; ability to work effectively in a team.
  • good organizational skills and ability to multitask
  • fluency in English is a must




These positions offer exciting opportunities for highly motivated individuals to contribute to advancing personalized medicine in cancer. We offer the opportunity to expand your portfolio with cutting-edge skills in 3D tumor model generation. We offer a stimulating and international research environment with a strong and dynamic network of international collaborators. The close collaboration with the IHB and clinicians opens up possibilities for direct translational applications and offers the candidates exposure to a major pharmaceutical company. We offer access to state-of-the-art computing and experimental infrastructure. Humanitas University offers dedicated training programs to broaden your expertise and enhance your skill set.