Laboratory Technician Molecular-Cell Biology

The research group of Precision Medicine and Oncognomics led by Prof. Salvatore Piscuoglio (salvatore.piscuoglio@hunimed.eu) will be based al the Humanitas University, Milan, Italy, from January 2024 is seeking three highly motivated Laboratory Technicians in Molecular Biology/ Cell Biology (100%) to join our team of bioinformaticians, molecular biologists, pathologists, and clinical fellows.

The candidates will develop, optimize and apply cutting-edge cellular/ molecular methods to generate 3D in vitro and ex vivo tumor models that adequately recapitulate the tumor architecture and mimic the cell-to-cell interactions within the tissue microenvironment. The specific tasks will include the generation and maintenance of patient-derived tumor organoids, patient-derived tumor fragments, fibroblasts, endothelial cells, Peripheral Blood Mononuclear Cells (PBMCs), and Tumor Infiltrating Lymphocytes (TILs). 

Main responsibilities include

• processing patient-derived samples according to pre-established guidelines (e.g., tissue dissociation, isolation of PBMCs/ TILs, endothelial cells, sample freezing and storage, nucleic acids and protein extraction, and patient-derived tumor organoid/ fragment generation and maintenance)
• generation and maintenance of cell cultures and execution of experiments on cell cultures
• updating the database of patient samples and reagents
• documenting the experiments
• providing general support for lab maintenance within the scope of this project

Profile

• "Laboratory Technician" degree or a Bachelor/Master degree in molecular biology, biotechnology or a related discipline
• excellent technical expertise in molecular/ cell biology techniques
• hands-on experience handling and processing patient samples
• hands-on experience with cell culture and flow cytometry techniques is a must
• experience in primary cell culture and 3D cultures is highly desirable
• technical expertise in organoid generation and maintenance is a plus
• once trained, the ability to work independently
• organized and detail-oriented; ability to maintain accurate documentation of experiments and sample information
• collaborative; ability to work effectively in a team.
• good organizational skills and ability to multitask
• fluency in English is a must

Offer

These positions offer exciting opportunities for highly motivated individuals to contribute to advancing personalized medicine in cancer. We offer the opportunity to expand your portfolio with cutting-edge skills in 3D tumor model generation. We offer a stimulating and international research environment with a strong and dynamic network of international collaborators. The close collaboration with the IHB and clinicians opens up possibilities for direct translational applications and offers the candidates exposure to a major pharmaceutical company. We offer access to state-of-the-art computing and experimental infrastructure. Humanitas University offers dedicated training programs to broaden your expertise and enhance your skill set.