Junior Staff Scientist_Electron Microscopy

Junior Staff scientist, Electron Microscopy

Humanitas (ICH, http://www.humanitas-research.org/) is a world-famous center of excellence for research and treatment of immune system-related diseases, from cancer to cardiovascular diseases and autoimmune diseases. In 2005, ICH has been acknowledged by the Italian Ministry of Health as a Clinical Institute for Care and Scientific Research (IRCCS). ICH is recognized for its research and management model. It is member of the European Network of Immunology Institutes. Being a clinical research hospital, the research activity is based on a strong collaboration between clinicians and researchers. A PhD program, accordant to the highest international standards, is active and provide daily laboratory research practice, together with courses on different disciplines. Scientific research is also integrated with the educational and training activity of the Humanitas University (https://www.hunimed.eu), an international university dedicated to the life sciences.

The CLEM Core (https://www.humanitas-research.com/tech-platforms/clem-core/) provides the state of the art of electron and light microscopy imaging for investigations at the interface between basic and clinical research. The CLEM Core technology and expertise allow researchers to probe a wide range of biological samples, biocompatible and inorganic materials with a continuum resolution from tens of millimeters to nanometer scale. The CLEM Core leverages fluorescence live cell imaging, confocal microscopy, cryoEM, STEM, volume electron microscopy (vEM) by FIB-SEM and array tomography. CLEM Core has access to the Humanitas University High-performance computing cluster and a dedicated high-performance computing node for data analysis. The personnel of the facility are involved in scientific (external and internal) collaborations for the development of CLEM-based applications for preclinical and clinical research.

Junior Staff scientist, Electron Microscopy

The successful candidate will support routine EM operations and contribute to workflow development within a multidisciplinary imaging platform. Prior exposure in EM sample preparation is mandatory. The selected candidate will receive advanced training in High-Pressure Freezing (HPF), cryoCLEM, array tomography, and FIB-SEM.

The person filling this position will:

• Maintain daily instruments.

• Develop new hardware and experimental pipelines.

• Provide design support for experiments.
• Provide sample preparation and acquisition service or assistance.
• Train and support users.

You’re the perfect candidate if you have:

• Proven experience of 3 years post-degree (M.Sc.) research experience in Life Sciences, Biology, Biotechnology, Biochemistry, or related field
• At least 1 year of documented hands-on experience with electron microscopy
• Basic experience with scripting languages (python/bash/R) or with using the command line.

Additional requirements we are looking for:

• Experience in cryoEM techniques.
• Experience with live cell imaging and fluorescence microscopy.

All candidate data collected from the application shall be processed in accordance with applicable law: Dlgs 198/2006 e dei Dlgs 215/2003 e 216/2003;  privacy ex artt. 13 e 14 del Reg. UE 2016/679.